利用壓力循環技術（PCT）從植物組織中提取RNA

發表時間：2009/2/27

Extraction of nucleic acids from plants is a time consuming and labor intensive process. Two common methods of

extraction are （i） grinding individual samples with mortar and pestle, and （ii） mechanical mincing. Unfortunay,

these methods are prone to contamination, as the same tools are often reused sequentially for multiple

preparations. This risk of contamination is often unacceptable, especially in studies using rare or valuable

specimens, such as when breeding selection is critical （particularly with the advent of genetically

engineered crops）, or when inlectual property discovery or protection is involved. Here we compare

cell isruption by Qiagen RNeasy with mortar and pestle to pressure cycling technology PCT） for the

extraction of RNA from corn sprouts, grape seeds, and grape skin.

利用壓力循環技術（PCT）從植物組織中提取RNA
從植物中提取核酸是一件非常耗時耗力的工作。目前常見的提取方法有人工研磨和機械破碎兩種方法。因為研磨工具和破碎刀頭經常反復使用，所以這兩種方法非常容易造成污染。在對非常或者非常有價值的樣品進行研究時，污染常常不能被接受。本文介紹了利用壓力循環技術（PCT）和傳統研磨技術兩種不同的方法從玉米芽、葡萄種子和葡萄皮中提取RNA，并對這兩種方法進行了比較。

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